This film was made using a Swift FM-31 Field Microscope and Sony DSC-W7 Cybershot Camera, viewing a sample taken from a San Francisco Bay Salt Marsh pond at 800X magnification. The "snake-like" critters are Spirochete bacteria and the "boat-shaped" ones are Pennate Diatoms. Diatoms are algae in glass [silica] shells. That means they are tiny photosynthetic plants, using sunlight for energy. The role of the Spirochetes in this pond community is not known. Of the many millions of species of bacteria, only a very few are pathogens and even fewer are human pathogens. These little guys probably make their living digesting the remains of plant and animal material in the pond. The water in salt marsh ponds is often very salty, being as much as 5% salt.
Video of spirochetes. http://www.lyme-disease-research-data... The Lyme Disease Research Database is an on going database of lyme research, articles, news, therapies and lifestyle support resources.
Spirochete as imaged using CytoViva's enhanced resolution capabilities
Normal textbook morphology of Borrilia Burgdorferi 31. Six spirals long. Motal. Actual speed not sped up. This boy moves. Nonviscous fluid doesn't allow the coils to really grab anything, but the bug does manage to cross the field of view. A recurring theme is the very little jiggly guys that move around in all of these Bb31 monocultures. I know, Normal textbook morphology of Borrilia Burgdorferi 31. Six spirals long. Motal. Actual speed not sped up. This boy moves. Nonviscous fluid doesn't allow the coils to really grab anything, but the bug does manage to cross the field of view. A recurring theme is the very little jiggly guys that move around in all of these Bb31 monocultures. I know, I know. "Brownian motion". That explains the random movements but immunoflurecent staining shows objects this size that stain positive. One shows up in the last few seconds of this movie.
Two large "double spirochetes" Bb31 in BSKII agar with penicillin solution wicked across live slide prep.
Lower right bugs. A perfect double spirochete. Double only forms one "cyst". Not sure why, maybe because they are still attached. If separated I think they would form two. Depending on which side of the lyme controversy you stand on. Dead bug or Dormant bug.
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